Permeabilization of Drosophila embryos for introduction of small molecules

A method of permeabilization of Drosophila embryos for assays of small molecule activity.

The Drosophila embryo has long been a powerful laboratory model for elucidating molecular and genetic mechanisms that control development. The ease of genetic manipulations with this model has supplanted pharmacological approaches that are commonplace in other animal models and cell-based assays. Here we describe recent advances in a protocol that enables application of small molecules to the d...

INTERACTION OF PROTEINS WITH SMALL MOLECULES

Permeabilization of Cochliomyia hominivorax (Diptera: Calliphoridae) embryos.

Embryos of the primary screwworm, Cochliomyia hominivorax (Coquerel), were successfully permeabilized for use in subsequent cryopreservation studies. Mortality was greater for eggs incubated for < 5 h before treatment. The mean survival of embryos to first instars was 55.7, 61.1, and 62.6% when the embryos were incubated for 5, 5.5, and 6 h before treatment, respectively. The survival to the pu...

study of cohesive devices in the textbook of english for the students of apsychology by rastegarpour

this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...

Transient expression of DNA after ballistic introduction into Drosophila embryos.

Current procedures for introduction of DNA into Drosophila embryos require the time-consuming microinjection of DNA solution into individual embryos (1, 2). A procedure has recently been used successfully in plants, yeast and cultured cells for the ballistic introduction of DNA via tungsten (W) particles (3-5) . Using a modification of this procedure for Drosophila embryos, we have obtained tra...